leipner
photosynthesis
consulting

leipner photosynthesis consulting

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leipner photosynthesis consulting
Dr. Jörg Leipner
Neumattstrasse 2
4450 Sissach
SWITZERLAND

email: leipner@photosynthesis.ch
mobile: +41 78 817 3559

Applications

Screening for chilling tolerance of maize seedlings

The possibility of using chlorophyll fluorescence technique as a selection tool for improving the chilling tolerance of maize was investigated in six genotypes differing greatly in their chilling tolerance (Fracheboud et al., 1999). Upon gradual cooling measurements of the operating quantum efficiency of PSII photochemistry (Fq'/Fm') indicated that leaves of tolerant genotypes, that developed at suboptimal temperature, maintained higher rates of electron transport than leaves of sensitive genotypes. Applying the method to flint and dent breeding population led to a substantial increase (up to 31%) in the photosynthetic capacity of hybrids between selected F3 inbreeding families grown at suboptimal temperature, demonstrating that the method is an efficient selection tool for improving the chilling tolerance of maize through breeding.

Imbred lines of contrasting chilling tolerance that were developed by this method were used to investigate the genetic basis of chilling tolerance under controlled as well as under field conditions by analyzing quantitative trait loci (QTL) of an F2:3 population (Fracheboud et al., 2004; Jompuk et al., 2005; Leipner et al., 2008).

Marker and phenotype assisted backcrossing have successfully improved the chilling tolerance of experimental lines (PhD thesis S.K. Biradar) and lines that are used for hybrid production (business report 2003/04 of Delley Samen und Pflanzen AG).

Screening maize seedlings for chilling tolerance of photosynthesis
Screening maize seedlings for chilling tolerance of photosynthesis

 

Chlorophyll fluorescence measurements in winter wheat

examples

Screening for chilling tolerance of maize seedlings
[further information]

Discriminating winter wheat for frost tolerance
[further information]

Detecting ozone damage in Phaseolus
[further information]

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